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"article": {
"title": "In vitro regeneration of some promising genotypes of sugarcane (Saccharum Officinarum L.) in Bangladesh",
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"article": {
"title": "Effect of growth regulators concentrations on in vitro multiplication of three elite sugarcane (Saccharum officinarum L.) Genotypes using shoot tip culture",
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"article": {
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"article": {
"title": "IN VITRO MICROPROPAGATION STUDIES IN SUGARCANE",
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"title": "Direct plant regeneration through micropropagation using selected explants of sugarcane",
"journal": "International Journal of Advanced Geosciences",
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"section": "abstract",
"article": {
"title": "DIRECT REGENERATION OF SUGARCANE PLANTLETS: A TOOL TO UNRAVEL GENETIC HETEROGENEITY",
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"year": 2009,
"doi": "10.13140/rg.2.2.28819.22563",
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"article": {
"title": "Optimized Micropropagation Protocol and Genetic Fidelity Assessment of Sugarcane (Saccharum spp. Hybrid) Variety CoLk 11206 using ISSR and SSR Markers",
"journal": "International Journal of Agricultural and Statistical Sciences",
"year": 2026,
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"section": "abstract",
"article": {
"title": "Optimized Micropropagation Protocol and Genetic Fidelity Assessment of Sugarcane (Saccharum spp. Hybrid) Variety CoLk 11206 using ISSR and SSR Markers",
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"text_level": "abstract",
"section": "abstract",
"article": {
"title": "Regeneration of industrial sugarcane using in-vitro plant apical meristem",
"journal": "Electronic Journal of Plant Breeding",
"year": 2019,
"doi": "10.5958/0975-928x.2018.00167.9",
"pmid": null,
"pmcid": null
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}
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{
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"sentence": "The highest number of multiple shoots was produced on MS medium fortified with KIN 1.0 mg/l.",
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"section": "abstract",
"article": {
"title": "An efficient protocol optimization for callus induction, regeneration and acclimatization in sugarcane cv. CO 94012",
"journal": "The Pharma Innovation",
"year": 2022,
"doi": "10.22271/tpi.2022.v11.i4n.11969",
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{
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"text_level": "abstract",
"section": "abstract",
"article": {
"title": "Thidiazuron-induced rapid shoot regeneration via embryo-like structure formation from shoot tip-derived callus culture of sugarcane",
"journal": "Plant Biotechnology",
"year": 2010,
"doi": "10.5511/plantbiotechnology.27.365",
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{
"id": 149,
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"carbon": null,
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{
"id": "BAP",
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"sentence": "Maximum shoots proliferation was observed on MS medium containing 1mg/l BA and 0.8mg/l NAA.",
"text_level": "abstract",
"section": "abstract",
"article": {
"title": "New approaches for reducing the cost of the synthetic seeds storage using sugarcane bagasse and different additives to the gel matrix for sugarcane plant in vitro.",
"journal": "Egyptian Journal of Botany",
"year": 2018,
"doi": "10.21608/ejbo.2017.1747.1122",
"pmid": null,
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}
},
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"id": 150,
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"sentence": "The results indicated that 0.5 mg/L BAP without NAA produced the most effective response for axillary shoot initiation, as reflected by higher explant survival and improved shoot growth compared to other treatments.",
"text_level": "abstract",
"section": "abstract",
"article": {
"title": "In Vitro Axillary Shoot Growth of Sugarcane Cultivar ‘AAS Agribun’ under BAP and NAA",
"journal": "BIOTIK Jurnal Ilmiah Biologi Teknologi dan Kependidikan",
"year": 2026,
"doi": "10.22373/biotik.v14i2.35501",
"pmid": null,
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}
},
{
"id": 122,
"type": "protocol",
"stage": "somatic_embryogenesis",
"explant": null,
"basal_medium": "MS",
"medium_strength": 1,
"carbon": {
"source": "sucrose",
"g_per_l": 30
},
"growth_regulators": [
{
"id": "24D",
"name": "2,4-D",
"mg_per_l": 4,
"as_reported": "4 ppm"
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"sentence": "The results are showed that the optimal induction of embryogenic callus on medium MS + sucrose 30 g / L + CH 300 ppm + 300 ppm PVP + 2,4-D 4 ppm as indicated by the high percentage of explants forming embryogenic callus that is equal to 40% and the respective concentration 2 ppm and 3 ppm is 33,3% and 37,5%.",
"text_level": "abstract",
"section": "abstract",
"article": {
"title": "Induction Somatic Embryogenesis Used 2,4-Dichlorophenoxyacetic Acid (2,4-D) and Kinetin in Spindle Leaf Explant Sugarcane",
"journal": "Jurnal ILMU DASAR",
"year": 2015,
"doi": "10.19184/jid.v16i1.1387",
"pmid": null,
"pmcid": null
}
}
},
{
"id": 43,
"type": "sterilization",
"stage": "sterilization",
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"basal_medium": null,
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"carbon": null,
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"reagent": "HgCl2",
"conc_pct": 0.1,
"sec_min": 1800,
"sec_max": 1800
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"review": "machine-checked",
"evidence": {
"sentence": "The shoot tops were then surface sterilized using Bavistin™ (0.1%) and streptomycin (0.1%) for 15 min followed by HgCl2 (0.1%) for 30 min and then rinsed with sterile distilled water for 2–3 times.",
"text_level": "fulltext",
"section": "methods",
"article": {
"title": "Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers.",
"journal": "3 Biotech",
"year": 2017,
"doi": "10.1007/s13205-016-0579-3",
"pmid": "28391480",
"pmcid": "PMC5385170"
}
}
},
{
"id": 71,
"type": "sterilization",
"stage": "sterilization",
"explant": "axillary bud",
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"medium_strength": null,
"carbon": null,
"growth_regulators": [],
"response_pct": null,
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"reagent": "HgCl2",
"conc_pct": 0.1,
"sec_min": 420,
"sec_max": 420
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},
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"review": "machine-checked",
"evidence": {
"sentence": "The rapid micropropagation technique of sugarcane variety YT55 was studied.Lateral buds as explants source were disinfected by 0.1% solution of mercuric chloride for 7 minutes,then to plant in MS medium with 1.0 mg/L 6-BA and 0.2 mg/L NAA for shoot inducing.The results showed that lots of plantlets were induced.MS was the optimum medium with 1.0 mg/L 6-BA and the medium with 2.0 mg/L NAA for rapid micropropagation and rooting response,respectively.The survival rate was between 90% and 95% when the sugarcane plantlets were transplanted in the medium of wood chaff.",
"text_level": "abstract",
"section": "methods",
"article": {
"title": "Rapid Micropropagation of Sugarcane(Saccharum officinarum L.) Variety YT55",
"journal": "Zhongguo tangliao",
"year": 2012,
"doi": null,
"pmid": null,
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}
},
{
"id": 83,
"type": "sterilization",
"stage": "sterilization",
"explant": null,
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"medium_strength": null,
"carbon": null,
"growth_regulators": [],
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"conc_pct": 0.1,
"sec_min": 600,
"sec_max": 600
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"aseptic_pct": null,
"substrate": null,
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"papers_agreeing": 1,
"review": "machine-checked",
"evidence": {
"sentence": "Surface sterilization with 0.1% mercury chloride for 10 min was used as standard check.",
"text_level": "abstract",
"section": "methods",
"article": {
"title": "An alternative safer and cost effective surface sterilization method for sugarcane (Saccharum officinarum L.) explants",
"journal": "AFRICAN JOURNAL OF BIOTECHNOLOGY",
"year": 2013,
"doi": "10.5897/ajb2013.12481",
"pmid": null,
"pmcid": null
}
}
},
{
"id": 84,
"type": "sterilization",
"stage": "sterilization",
"explant": "shoot tip",
"basal_medium": null,
"medium_strength": null,
"carbon": null,
"growth_regulators": [],
"response_pct": null,
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"time_days": null,
"sterilization": {
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"aseptic_pct": null,
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"review": "machine-checked",
"evidence": {
"sentence": "The study verified that surface sterilization with Berekina 5% ingredients of chlorine for 25 min exposure time is optimal for sugarcane shoot tip decontamination and this treatment combination can replace sterilization with 0.1% mercury chloride for 10 min. Key words: Berekina, mercury chloride, exposure time, shoot tip, in vitro.",
"text_level": "abstract",
"section": "methods",
"article": {
"title": "An alternative safer and cost effective surface sterilization method for sugarcane (Saccharum officinarum L.) explants",
"journal": "AFRICAN JOURNAL OF BIOTECHNOLOGY",
"year": 2013,
"doi": "10.5897/ajb2013.12481",
"pmid": null,
"pmcid": null
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}
},
{
"id": 97,
"type": "sterilization",
"stage": "sterilization",
"explant": null,
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"reagent": "ethanol",
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{
"reagent": "NaOCl",
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}
],
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"section": "methods",
"article": {
"title": "Evaluation of organic vinegar treatments and black gram [Vigna mungo (L.) Hepper] genotypes for the management of pod rot disease caused by Fusarium humuli.",
"journal": "Frontiers in plant science",
"year": 2026,
"doi": "10.3389/fpls.2026.1647478",
"pmid": "41937764",
"pmcid": "PMC13045857"
}
}
},
{
"id": 98,
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{
"reagent": "ethanol",
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{
"reagent": "NaOCl",
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}
],
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"sentence": "Under sterile conditions, the buds were disinfected by immersing them in 70% ethanol for 30 s, followed by immersion in a 10% sodium hypochlorite solution for 10 min. The buds were then rinsed 3–4 times with sterile water and dried using sterile filter paper.",
"text_level": "fulltext",
"section": "methods",
"article": {
"title": "Optimizing Sugarcane Clonal Propagation In Vitro by Using Calcium Ammonium Nitrate and Ammonium Sulfate.",
"journal": "Plants (Basel, Switzerland)",
"year": 2024,
"doi": "10.3390/plants13192767",
"pmid": "39409637",
"pmcid": "PMC11478678"
}
}
},
{
"id": 100,
"type": "sterilization",
"stage": "sterilization",
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"steps": [
{
"reagent": "bleach",
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"evidence": {
"sentence": "The explants were surface sterilized with 50% clorox for 30 minutes.",
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"section": "methods",
"article": {
"title": "Effect of cytokinins on shoot multiplication in three elite sugarcane varieties",
"journal": "Pakistan Journal of Botany",
"year": 2009,
"doi": null,
"pmid": null,
"pmcid": null
}
}
},
{
"id": 103,
"type": "sterilization",
"stage": "sterilization",
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"steps": [
{
"reagent": "ethanol",
"conc_pct": 75,
"sec_min": 30,
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{
"reagent": "NaOCl",
"conc_pct": 5,
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],
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"sentence": "For isolation, leaf tissues at the junction of diseased and healthy regions were cut into approximately 0.3 cm × 0.3 cm squares and soaked in 75% alcohol for 30 s, followed by surface sterilization in 5% NaClO for 4 min, rinsed 3 times with sterile water, then placed on a sterilized filter paper to remove residual solution ().",
"text_level": "fulltext",
"section": "methods",
"article": {
"title": "Pathogen identification and fungicide screening of Sugarcane White Rash Disease in China.",
"journal": "Frontiers in microbiology",
"year": 2026,
"doi": "10.3389/fmicb.2026.1860711",
"pmid": "42440825",
"pmcid": "PMC13334388"
}
}
},
{
"id": 128,
"type": "sterilization",
"stage": "sterilization",
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"steps": [
{
"reagent": "NaOCl",
"conc_pct": 4,
"sec_min": 3600,
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},
{
"reagent": "ethanol",
"conc_pct": 95,
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],
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"evidence": {
"sentence": "Riceberry rice seeds (Oryza sativa) were surface-sterilised with 4% NaOCl in an orbital shaker for 1 h and rinsed with sterile distilled water. Seeds were immersed in a 95% ethanol solution for 5 min. Afterwards, the seeds were washed several times with autoclaved double distilled water ().",
"text_level": "fulltext",
"section": "methods",
"article": {
"title": "Characterisation of Plant Growth-Promoting Endophytic Bacteria from Sugarcane and Their Antagonistic Activity against Fusarium moniliforme.",
"journal": "Tropical life sciences research",
"year": 2021,
"doi": "10.21315/tlsr2021.32.3.6",
"pmid": "35656370",
"pmcid": "PMC9132556"
}
}
}
]
}