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Cultivar: Mareko Fana", "journal": "American Journal of Plant Sciences", "year": 2015, "doi": "10.4236/ajps.2015.69143", "pmid": null, "pmcid": null } } }, { "id": 205, "type": "protocol", "stage": "shoot", "explant": "cotyledon", "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [ { "id": "GA3", "name": "GA3", "mg_per_l": 0.5, "as_reported": "0.5 mg/L" } ], "response_pct": null, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": null, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "Shoot regeneration was most effective with 5 mg/L AgNO3 and 1 nM CaREF1 for cotyledon explants, and shoot elongation was enhanced with GA3 (0.5 mg/L) and CaREF1 (1 nM) in Zunla-1.", "text_level": "abstract", "section": "abstract", "article": { "title": "An optimized regeneration protocol for chili peppers (Capsicum annuum L.) through genotype-specific explant and growth regulator combinations", "journal": "Seed Biology", "year": 2025, "doi": "10.48130/seedbio-0025-0012", "pmid": null, "pmcid": null } } }, { "id": 206, "type": "protocol", "stage": "shoot", "explant": null, "basal_medium": "MS", "medium_strength": 1, "carbon": null, "growth_regulators": [ { "id": "BAP", "name": "BAP", "mg_per_l": 2, "as_reported": "2.0 mg/l" }, { "id": "IAA", "name": "IAA", "mg_per_l": 0.5, "as_reported": "0.5 mg/l" } ], "response_pct": null, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": null, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "The highest number of regenerated shoot buds was obtained when shoot explants were cultured on a MS medium supplemented with 2.0 mg/l BAP and 0.5 mg/l IAA.", "text_level": "abstract", "section": "abstract", "article": { "title": "Direct regeneration protocols of five Capsicum annuum L. varieties", "journal": "AFRICAN JOURNAL OF BIOTECHNOLOGY", "year": 2014, "doi": "10.5897/ajb2013.12592", "pmid": null, "pmcid": null } } }, { "id": 211, "type": "protocol", "stage": "shoot", "explant": "cotyledon", "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [ { "id": "ZEA", "name": "Zeatin", "mg_per_l": 2, "as_reported": "2 mg/l" }, { "id": "IAA", "name": "IAA", "mg_per_l": 1, "as_reported": "1 mg/l" } ], "response_pct": 92, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": null, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "The best combination so far was 2 mg/l of zeatin and 1 mg/l of IAA, resulting in 92% of regeneration from cotyledon explant.", "text_level": "abstract", "section": "abstract", "article": { "title": "EFFECT OF EXPLANTS AND GROWTH REGULATORS ON SOMATIC EMBRYO-GENESIS AND ADVENTITIOUS ORGANOGENESIS IN CAPSICUM ANNUUM", "journal": "HortScience", "year": 1992, "doi": "10.21273/hortsci.27.6.618c", "pmid": null, "pmcid": null } } }, { "id": 212, "type": "sterilization", "stage": "sterilization", "explant": null, "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [], "response_pct": null, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": { "steps": [ { "reagent": "NaOCl", "conc_pct": 1, "sec_min": 600, "sec_max": 600 } ], "additives": [] }, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "The seeds were sterilized with 1% (v/v) NaClO for 10 min, rinsed with deionized water five times, transferred to 200-well trays containing substrate (peat: vermiculite: perlite = 3:1:1, Shouguang Hengxian Agricultural Science and Technology Co., Ltd., Xingyuan Road, Gucheng Sub-district, Shouguang, Shandong Province, China), and then grown in a growth chamber under a 16 h/8 h (day/night) photoperiod at 28 °C/22 °C with a relative humidity was maintained at 60%.", "text_level": "fulltext", "section": "methods", "article": { "title": "Prohexadione calcium boosts pepper stem rigidity: coordinated regulation of hormonal crosstalk and lignin deposition drives xylem reinforcement.", "journal": "BMC plant biology", "year": 2025, "doi": "10.1186/s12870-025-07255-6", "pmid": "41016936", "pmcid": "PMC12477794" } } } ] }