{ "generated_at": "2026-10-17T04:51:45+00:00", "generator": "TissueScope 1.0.0", "notice": "Machine-extracted from published literature; every record carries its source sentence. Verify against the cited paper before use.", "plant": { "scientific_name": "Abelmoschus esculentus", "authority": "Abelmoschus esculentus Moench, 1794", "family": "Malvaceae", "common_names": "okra; lady's fingers; lady's-fingers; quiabo; Antsigini gombo; Giambo; Lady's-Finger; Ochro", "ncbi_taxid": "455045", "gbif_key": "3152707", "last_scanned": "2026-10-16 21:08:46" }, "ranked_protocols": { "shoot": [ { "rank": 1, "close_competitor": false, "papers": 1, "corroborated": false, "score_pct_of_leader": 100, "growth_regulators": [ { "id": "TDZ", "raw": "0.8", "unit": "mg/L", "mgl": 0.8 } ], "explant": null, "basal_medium": null, "medium_strength": null, "response_pct_median": null, "shoots_per_explant_median": 27.04, "record_ids": [ 3 ] }, { "rank": 2, "close_competitor": false, "papers": 1, "corroborated": false, "score_pct_of_leader": 67, "growth_regulators": [ { "id": "IBA", "raw": "1.0", "unit": "mg/L", "mgl": 1 }, { "id": "NAA", "raw": "0.5", "unit": "mg/L", "mgl": 0.5 } ], "explant": "shoot tip", "basal_medium": null, "medium_strength": null, "response_pct_median": null, "shoots_per_explant_median": null, "record_ids": [ 1 ] }, { "rank": 3, "close_competitor": false, "papers": 1, "corroborated": false, "score_pct_of_leader": 67, "growth_regulators": [ { "id": "BAP", "raw": "2.0", "unit": "mgL-1", "mgl": 2 } ], "explant": null, "basal_medium": null, "medium_strength": null, "response_pct_median": null, "shoots_per_explant_median": null, "record_ids": [ 7 ] }, { "rank": 4, "close_competitor": false, "papers": 1, "corroborated": false, "score_pct_of_leader": 66, "growth_regulators": [ { "id": "KIN", "raw": "0.5", "unit": "mg/L", "mgl": 0.5 } ], "explant": null, "basal_medium": "MS", "medium_strength": 1, "response_pct_median": null, "shoots_per_explant_median": null, "record_ids": [ 2 ] } ], "rooting": [ { "rank": 1, "close_competitor": false, "papers": 1, "corroborated": false, "score_pct_of_leader": 100, "growth_regulators": [ { "id": "IBA", "raw": "2.0", "unit": "mgL-1", "mgl": 2 } ], "explant": null, "basal_medium": null, "medium_strength": null, "response_pct_median": 79, "shoots_per_explant_median": null, "record_ids": [ 8 ] } ] }, "sterilization": [ { "key": "18484a22998a05783cf6c86eaee05b2f8efc6eaf", "text": "5% NaOCl · 10 min", "explants": [], "n_papers": 1, "score": 1.24, "aseptic": null, "corroborated": false, "confirmed_n": 0, "evidence": [ 6 ], "steps": [ { "reagent": "NaOCl", "conc_pct": 5, "sec_min": 600, "sec_max": 600 } ], "rank": 1, "leader": true, "close": false, "score_pct": 100 }, { "key": "a29efc5c74573c2e288c3d1d866d7ec6ebedeece", "text": "1% NaOCl · 15 min", "explants": [], "n_papers": 1, "score": 1.232, "aseptic": null, "corroborated": false, "confirmed_n": 0, "evidence": [ 9 ], "steps": [ { "reagent": "NaOCl", "conc_pct": 1, "sec_min": 900, "sec_max": 900 } ], "rank": 2, "leader": false, "close": true, "score_pct": 99 }, { "key": "51b45d56db4bcd239c767b586d0c7f44d4135550", "text": "20% commercial bleach · 10 min → 10% commercial bleach · 10 min", "explants": [], "n_papers": 1, "score": 0.888, "aseptic": null, "corroborated": false, "confirmed_n": 0, "evidence": [ 10 ], "steps": [ { "reagent": "bleach", "conc_pct": 20, "sec_min": 600, "sec_max": 600 }, { "reagent": "bleach", "conc_pct": 10, "sec_min": 600, "sec_max": 600 } ], "rank": 3, "leader": false, "close": false, "score_pct": 72 } ], "acclimatization": [ { "key": "118e80c094833aaf1312fddfc5f9433d88b0a4a8", "substrate": "soil", "survival": 100, "min": 100, "max": 100, "n_papers": 1, "score": 1.297, "corroborated": false, "confirmed_n": 0, "evidence": [ 4 ], "rank": 1, "leader": true, "close": false, "score_pct": 100 } ], "records": [ { "id": 4, "type": "acclimatization", "stage": "acclimatization", "explant": null, "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [], "response_pct": null, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": null, "aseptic_pct": null, "substrate": "soil", "survival_pct": 100, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "Regenerated plantlets were successfully transferred to soil, with a 100% survival rate and no differences in morphology or growth characteristics com-pared to control plants.", "text_level": "abstract", "section": "abstract", "article": { "title": "In vitro Regeneration of Multiple Shoots in Abelmoschus esculentus (L.) Moench (Okra) via Apical Shoot Meristem Culture", "journal": "Current Biotechnology", "year": 2023, "doi": "10.2174/0122115501261522231013100629", "pmid": null, "pmcid": null } } }, { "id": 8, "type": "protocol", "stage": "rooting", "explant": null, "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [ { "id": "IBA", "name": "IBA", "mg_per_l": 2, "as_reported": "2.0 mgL-1" } ], "response_pct": 79, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": null, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "Healthy and strong roots (79%) were obtained on media containing 2.0 mgL−1 IBA and 100 mgL−1 AC.", "text_level": "abstract", "section": "abstract", "article": { "title": "Role of reduced nitrogen for induction of embryogenic callus induction and regeneration of plantlets in Abelmoschus esculentus L.", "journal": "South African Journal of Botany", "year": 2020, "doi": "10.1016/j.sajb.2020.01.016", "pmid": null, "pmcid": null } } }, { "id": 1, "type": "protocol", "stage": "shoot", "explant": "shoot tip", "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [ { "id": "IBA", "name": "IBA", "mg_per_l": 1, "as_reported": "1.0 mg/L" }, { "id": "NAA", "name": "NAA", "mg_per_l": 0.5, "as_reported": "0.5 mg/L" } ], "response_pct": null, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": null, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "Combination of 1.0 mg/L IBA and 0.5 mg/L NAA were found to be most effective for plant regeneration from apical shoot.", "text_level": "abstract", "section": "abstract", "article": { "title": "Regeneration of okra ( Abelmoschus esculentus L.) via apical shoot culture system", "journal": "AFRICAN JOURNAL OF BIOTECHNOLOGY", "year": 2012, "doi": null, "pmid": null, "pmcid": null } } }, { "id": 2, "type": "protocol", "stage": "shoot", "explant": null, "basal_medium": "MS", "medium_strength": 1, "carbon": null, "growth_regulators": [ { "id": "KIN", "name": "Kinetin", "mg_per_l": 0.5, "as_reported": "0.5 mg/L" } ], "response_pct": null, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": null, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "Best shoot elongation observed in MS medium supplemented with kinetin 0.5 mg/L.", "text_level": "abstract", "section": "abstract", "article": { "title": "Regeneration of okra ( Abelmoschus esculentus L.) via apical shoot culture system", "journal": "AFRICAN JOURNAL OF BIOTECHNOLOGY", "year": 2012, "doi": null, "pmid": null, "pmcid": null } } }, { "id": 3, "type": "protocol", "stage": "shoot", "explant": null, "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [ { "id": "TDZ", "name": "TDZ", "mg_per_l": 0.8, "as_reported": "0.8 mg/L" } ], "response_pct": null, "shoots_per_explant": 27.04, "roots_per_shoot": null, "time_days": null, "sterilization": null, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "Results: The highest number of multiple shoots (27.04 shoots) was obtained with 0.8 mg/L TDZ.", "text_level": "abstract", "section": "abstract", "article": { "title": "In vitro Regeneration of Multiple Shoots in Abelmoschus esculentus (L.) Moench (Okra) via Apical Shoot Meristem Culture", "journal": "Current Biotechnology", "year": 2023, "doi": "10.2174/0122115501261522231013100629", "pmid": null, "pmcid": null } } }, { "id": 7, "type": "protocol", "stage": "shoot", "explant": null, "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [ { "id": "BAP", "name": "BAP", "mg_per_l": 2, "as_reported": "2.0 mgL-1" } ], "response_pct": null, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": null, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "Result: The best shoot proliferation was obtained in the medium supplemented with 2.0 mgL-1 of BAP.", "text_level": "abstract", "section": "abstract", "article": { "title": "Effects of Benzylaminopurine on in vitro Proliferation and Shoot Growth of Okra [Abelmoschus esculentus (L.) Moench]", "journal": "Legume Research - An International Journal", "year": 2026, "doi": "10.18805/lrf-912", "pmid": null, "pmcid": null } } }, { "id": 6, "type": "sterilization", "stage": "sterilization", "explant": null, "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [], "response_pct": null, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": { "steps": [ { "reagent": "NaOCl", "conc_pct": 5, "sec_min": 600, "sec_max": 600 } ], "additives": [] }, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "Initially, the seeds were disinfected in a 5% sodium hypochlorite solution for 10 min, then rinsed several times with tap water and once with distilled water.", "text_level": "fulltext", "section": "methods", "article": { "title": "Protective role of Salicylic acid and sodium nitroprusside foliar application against copper stress in okra (Abelmoschus esculentus).", "journal": "Scientific reports", "year": 2025, "doi": "10.1038/s41598-025-17608-8", "pmid": "40890339", "pmcid": "PMC12402310" } } }, { "id": 9, "type": "sterilization", "stage": "sterilization", "explant": null, "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [], "response_pct": null, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": { "steps": [ { "reagent": "NaOCl", "conc_pct": 1, "sec_min": 900, "sec_max": 900 } ], "additives": [] }, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "Healthy okra seeds were surface sterilized for 15 min with 1% (v/v) NaOCl, rinsed thoroughly 3 times with sterile distilled water, and dried under laminar airflow on sterile paper.", "text_level": "fulltext", "section": "methods", "article": { "title": "Effect of Metarhizium anisopliae (MetA1) on growth enhancement and antioxidative defense mechanism against Rhizoctonia root rot in okra", "journal": "Heliyon", "year": 2023, "doi": "10.1016/j.heliyon.2023.e18978", "pmid": "37636386", "pmcid": "PMC10450861" } } }, { "id": 10, "type": "sterilization", "stage": "sterilization", "explant": null, "basal_medium": null, "medium_strength": null, "carbon": null, "growth_regulators": [], "response_pct": null, "shoots_per_explant": null, "roots_per_shoot": null, "time_days": null, "sterilization": { "steps": [ { "reagent": "bleach", "conc_pct": 20, "sec_min": 600, "sec_max": 600 }, { "reagent": "bleach", "conc_pct": 10, "sec_min": 600, "sec_max": 600 } ], "additives": [] }, "aseptic_pct": null, "substrate": null, "survival_pct": null, "papers_agreeing": 1, "review": "machine-checked", "evidence": { "sentence": "This was achieved by first sterilizing with 20% Clorox for 10 minutes and then sterilizing them again with 10% Clorox for 10 minutes, resulting in a 96% sterility rate for the plant samples.", "text_level": "abstract", "section": "methods", "article": { "title": "ผลของสารควบคมการเจรญเตบโตจากแบคทเรยเอนโดไฟตตอการขยายพนธพชวงศขง ทหายากและมคณคาทางเศรษฐกจดวยเทคนคเพาะเลยงเนอเยอพช", "journal": "Srinakharinwirot University Journal of Sciences and Technology", "year": 2025, "doi": null, "pmid": null, "pmcid": null } } } ] }